GeneTex
United States (US)

HIF2 alpha antibody [ep190b]

Orthogonal
GTX30123_28_FACS_18121410_321.jpg
GTX30123_1337_WB_18121410_740.jpg
GTX30123_1336_WB_18121410_781.jpg
GTX30123_1609_IHC_18121410_416.jpg
GTX30123_1335_WB_18121410_183.jpg

Cat No. GTX30123

Host Mouse
Clonality Monoclonal
Clone Name ep190b
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, GSA, IHC
Reactivity Human, Mouse, Rat, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/ml
ICC/IF Assay dependent
IHC-P 1:150 - 1:300
IHC-Fr Assay dependent
FACS 1:400
IP Assay dependent
ELISA 1:100 - 1:2000
ChIP assay Assay dependent
GSA Assay dependent
IHC 1:150 - 1:300
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Specificity/Sensitivity

This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ECYT4 antibody , HIF2A antibody , HLF antibody , MOP2 antibody , PASD2 antibody , bHLHe73 antibody

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30123_28_FACS_18121410_321.jpg

GTX30123 FACS Image

FACS analysis of HepG2 cells using GTX30123 HIF2 alpha antibody [ep190b]. M1 is defined by unstained cells.
Purple : primary antibody
Dilution : 1:400

GTX30123_1337_WB_18121410_740.jpg

GTX30123 WB Image

WB analysis of multiple samples using GTX30123 HIF2 alpha antibody [ep190b].
Lane 1 and 5: HepG2 without Cobalt (II) Chloride
Lane 2 and 6: HepG2 with Cobalt (II) Chloride
Lane 3 and 7: HepG2 normoxic
Lane 4 and 8 : HepG2 hypoxic
Dilution : 1-2 μg/ml

GTX30123_1336_WB_18121410_781.jpg

GTX30123 WB Image

WB analysis of HeLa cell lysate using GTX30123 HIF2 alpha antibody [ep190b].

GTX30123_1609_IHC_18121410_416.jpg

GTX30123 IHC-P Image

IHC-P analysis of human heart tissue using GTX30123 HIF2 alpha antibody [ep190b].

GTX30123_1335_WB_18121410_183.jpg

GTX30123 WB Image

Orthogonal

WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b].
Lane 1: normoxic A549 lysate control
Lane 2: hypoxic A549 lysate

REFERENCE