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HIF2 alpha antibody [ep190b]

Orthogonal
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Flow cytometry (FACS). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in IHC (Paraffin sections) (IHC-P). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123
Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

Cat. No. GTX30123

Reference 8

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 ep190b

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IHC

Reactivity

 Human, Mouse, Rat, Hamster
Package
100 μl ($449)
See all HIF2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/ml
ICC/IF Assay dependent
IHC-P 1:150 - 1:300
FACS 1:400
IP Assay dependent
ELISA 1:100 - 1:2000
ChIP assay Assay dependent
Gel supershift assays Assay dependent
IHC 1:150 - 1:300
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Product Note

This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_386038

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73

Cellular Localization

Nucleus,Nucleus speckle

Background

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

Orthogonal

WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b].
Lane 1: normoxic A549 lysate control
Lane 2: hypoxic A549 lysate

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

WB analysis of multiple samples using GTX30123 HIF2 alpha antibody [ep190b].
Lane 1 and 5: HepG2 without Cobalt (II) Chloride
Lane 2 and 6: HepG2 with Cobalt (II) Chloride
Lane 3 and 7: HepG2 normoxic
Lane 4 and 8 : HepG2 hypoxic
Dilution : 1-2 μg/ml

Anti-HIF2 alpha antibody [ep190b] used in Flow cytometry (FACS). GTX30123

GTX30123 FACS Image

FACS analysis of HepG2 cells using GTX30123 HIF2 alpha antibody [ep190b]. M1 is defined by unstained cells.
Purple : primary antibody
Dilution : 1:400

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

WB analysis of HeLa cell lysate using GTX30123 HIF2 alpha antibody [ep190b].

Anti-HIF2 alpha antibody [ep190b] used in IHC (Paraffin sections) (IHC-P). GTX30123

GTX30123 IHC-P Image

IHC-P analysis of human heart tissue using GTX30123 HIF2 alpha antibody [ep190b].

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

Orthogonal

The data was published in the journal PLoS One in 2008. PMID: 19030229

Anti-HIF2 alpha antibody [ep190b] used in Western Blot (WB). GTX30123

GTX30123 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29045486

REFERENCE

Application Reference

MM Kiran et al. Medicine Science 2023; 12 (3) : 915-21 Hypoxia inducible factors and hypoxia associated factor expression profiles and prognostic significance in renal cell carcinomas.
Application : IHC-P
Reactivity : Human
Nagalakshmi VK et al. Clin Sci (Lond) 2018; 132 (23) : 2519-2545 Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction.
Reactivity : Mouse
Zera K et al. PLoS One 2017; 12 (10) : e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Chen S et al. Angiogenesis 2010; 13 (1) : 59-69 VHL and PTEN loss coordinate to promote mouse liver vascular lesions.
Application : IHC-P
Reactivity : Mouse
Wright TM et al. J Biol Chem 2010; 285 (17) : 12916-24 Identification of Ror2 as a hypoxia-inducible factor target in von Hippel-Lindau-associated renal cell carcinoma.
Application : WB
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REVIEW

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Application Reference
MM Kiran et al. Medicine Science 2023; 12 (3):915-21 Hypoxia inducible factors and hypoxia associated factor expression profiles and prognostic significance in renal cell carcinomas.
Application : IHC-P
Reactivity : Human
Nagalakshmi VK et al. Clin Sci (Lond) 2018; 132 (23):2519-2545 Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction.
Reactivity : Mouse
Zera K et al. PLoS One 2017; 12 (10):e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Chen S et al. Angiogenesis 2010; 13 (1):59-69 VHL and PTEN loss coordinate to promote mouse liver vascular lesions.
Application : IHC-P
Reactivity : Mouse
Wright TM et al. J Biol Chem 2010; 285 (17):12916-24 Identification of Ror2 as a hypoxia-inducible factor target in von Hippel-Lindau-associated renal cell carcinoma.
Application : WB
Myers K et al. PLoS Genet 2009; 5 (1):e1000324 ATR and Chk1 suppress a caspase-3-dependent apoptotic response following DNA replication stress.
Application : WB
Reactivity : Mouse
Hacker KE et al. PLoS One 2008; 3 (11):e3801 VHL type 2B mutations retain VBC complex form and function.
Application : WB
Catrina SB et al. Clin Cancer Res 2006; 12 (15):4506-14 hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by *-like growth factor-i.
Application : IHC
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449