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HIF2 alpha antibody

Orthogonal Overexpression
Anti-HIF2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30114
Anti-HIF2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30114
Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114
Anti-HIF2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30114
Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114
Anti-HIF2 alpha antibody used in Immunohistochemistry (IHC). GTX30114
Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114
Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114
Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114

Cat No. GTX30114

Reference 15
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat, Monkey, Fish
Package
100 μl ($319)
See all HIF2 alpha products
PRODUCT

Summary

HIF2 alpha antibody recognizes HIF2 alpha / EPAS1 protein, a transcription factor (predicted molecular weight of 97 kDa) induced by hypoxic conditions. It forms a heterodimer with HIF1 beta / ARNT to drive a specific gene expression profile in various physiological and pathological processes that occur in the context of hypoxia.

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:100-1:3000. Recognizes a band at 118kDa.
For IHC: Use at a dilution of 1:100.
For ICC/IF: Use at a dilution of 1:100-1:1000.
Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

97 kDa. ( Note )

Positive Control

Hypoxic lysates , HepG2 (1% O2 treatment for 24hr and using Urea buffer) , Myc-DDK human HIF2A-transfected 293T

Product Note

Specific for HIF-2 alpha/EPAS. Does not cross react with HIF-1 alpha.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A peptide derived from the mouse/human HIF2 alpha.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_370290

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endothelial PAS domain protein 1 , HIF-2alpha , HIF2A , HLF , HRF , MOP2 , bHLHe73

Background

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. (1)

Database

Research Area

DATA IMAGES

Anti-HIF2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30114

GTX30114 ICC/IF Image

HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-HIF2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30114

GTX30114 ICC/IF Image

HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114

GTX30114 WB Image

Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis on normoxic and hypoxic nuclear rat cell lysates.

Anti-HIF2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30114

GTX30114 ICC/IF Image

Immunocytochemistry/Immunofluorescence: HIF-2 alpha Antibody (GTX30114) - Detection of HIF-2 Alpha (Green) in RCC4 cells using GTX30114. Nuclei (Blue) are counterstained with Hoechst 33258.

Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114

GTX30114 WB Image

Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using GTX30114

Anti-HIF2 alpha antibody used in Immunohistochemistry (IHC). GTX30114

GTX30114 IHC Image

Immunohistochemistry: HIF-2 alpha Antibody (GTX30114) - Hif-2 alpha immunoreactivity in human cardiac myocytes

Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114

GTX30114 WB Image

GTX30114 utilized in western blot with PC12 nuclear extracts, 1:1,000, 5 second exposure.

Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114

GTX30114 WB Image

Orthogonal

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 0.01 O2 treatment for 24h) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114

GTX30114 WB Image

Overexpression

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Non-transfected (-) and HIF2A-transfected (+, including Myc-DDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Minuzzi LG et al. Cytokine 2021; 137 : 155306 Effects of short-term physical training on the interleukin-15 signalling pathway and glucose tolerance in aged rats.
Application : WB
Reactivity : Rat
Hoefflin R et al. Nat Commun 2020; 11 (1) : 4111 HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice.
Zhu S et al. J Agric Food Chem 2020; 68 (42) : 11726-11734 Collagen Hydrolysate Corrects Anemia in Chronic Kidney Disease via Anti-Inflammatory Renoprotection and HIF-2α-Dependent Erythropoietin and Hepcidin Regulation.
Application : WB
Reactivity : Mouse
Hoefflin R et al. Nat Commun 2020; 11 (1) : 4111 HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice.
Application : IHC-P
Reactivity : Mouse
Bobek J et al. Histochem Cell Biol 2019; Tuftelin and HIFs expression in osteogenesis.
Application : ICC/IF, IHC-P
Reactivity : Mouse
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Submit a Reference

REVIEW

HIF2 alpha antibody Cat No. GTX30114

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2014.PMID: 25010988
Anti-HIF2 alpha antibody used in Western Blot (WB). GTX30114
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017.PMID: 29137374
Submit a Review
Application Reference
Minuzzi LG et al. Cytokine 2021; 137:155306 Effects of short-term physical training on the interleukin-15 signalling pathway and glucose tolerance in aged rats.
Application : WB
Reactivity : Rat
Hoefflin R et al. Nat Commun 2020; 11 (1):4111 HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice.
Zhu S et al. J Agric Food Chem 2020; 68 (42):11726-11734 Collagen Hydrolysate Corrects Anemia in Chronic Kidney Disease via Anti-Inflammatory Renoprotection and HIF-2α-Dependent Erythropoietin and Hepcidin Regulation.
Application : WB
Reactivity : Mouse
Hoefflin R et al. Nat Commun 2020; 11 (1):4111 HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice.
Application : IHC-P
Reactivity : Mouse
Bobek J et al. Histochem Cell Biol 2019; Tuftelin and HIFs expression in osteogenesis.
Application : ICC/IF, IHC-P
Reactivity : Mouse
Tatli Dogan H et al. Diagn Pathol 2018; 13 (1):60 Prognostic significance of the programmed death ligand 1 expression in clear cell renal cell carcinoma and correlation with the tumor microenvironment and hypoxia-inducible factor expression.
Application : IHC-P
Reactivity : Human
Huang Y et al. Pulm Circ 2018;:2.05E+15 EXPRESS: Hypoxia inducible factor 2α (HIF2α/EPAS1) is associated with development of pulmonary hypertension in severe congenital diaphragmatic hernia patients.
Application : IHC-P
Reactivity : Human
Bharti SK et al. Oncotarget 2018; 9 (20):15326-15339 Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.
Application : WB
Reactivity : Human
Bin? L et al. Oncotarget 2017; 8 (48):83684-83697 Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.
Application : WB
Reactivity : Mouse
Ramchandani D et al. Lab Invest 2016; 96 (12):1234-1245 Activation of carbonic anhydrase IX by alternatively spliced tissue factor under late-stage tumor conditions.
Application : WB
Reactivity : Human
Kudova J et al. J Pineal Res 2016; Melatonin promotes cardiomyogenesis of embryonic stem cells via inhibition of HIF-1α stabilization.
Application : WB
Reactivity : Mouse
Smeyne M et al. Neuroscience 2015; 295:23-38 HIF1α is necessary for exercise-induced neuroprotection while HIF2α is needed for dopaminergic neuron survival in the substantia nigra pars compacta.
Application : ICC/IF
Reactivity : Mouse
Sun W et al. Cell Death Dis 2014; 5:e1322 Prolyl-4-hydroxylase 2 enhances hypoxia-induced glioblastoma cell death by regulating the gene expression of hypoxia-inducible factor-α.
Application : WB
Reactivity : Human
Burke B et al. J Pathol 2002; 196 (2):204-12 Expression of HIF-1alpha by human macrophages: implications for the use of macrophages in hypoxia-regulated cancer gene therapy.
Application : WB
Reactivity : Human
Ohh M et al. Nat Cell Biol 2000; 2 (7):423-7 Ubiquitination of hypoxia-inducible factor requires direct binding to the beta-domain of the von Hippel-Lindau protein.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319