GeneTex
  • Country / Location Selection

United States (US)

HIF3 alpha antibody

Anti-HIF3 alpha antibody used in Western Blot (WB). GTX132071

Cat No. GTX132071

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

HIF3A-transfected 293T cells
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HIF3 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

hypoxia inducible factor 3 subunit alpha,HIF-3A,HIF3-alpha-1,IPAS,MOP7,PASD7,bHLHe17

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HIF3 alpha antibody used in Western Blot (WB). GTX132071

GTX132071 WB Image

HIF3 alpha antibody detects HIF3 alpha protein by western blot analysis. Non-transfected (-) and HIF3 alpha-transfected (+, inculding Myc / DDK-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF3 alpha antibody (GTX132071) diluted at 1:5000.

REFERENCE
There are currently no references for HIF3 alpha antibody (GTX132071). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HIF3 alpha antibody (GTX132071). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169