GeneTex
United States (US)

HIF3 alpha antibody

HIF3A-antibody-GTX85702-IHC-1_18121411_666.jpg
HIF3A-antibody_WB_GTX85702-2_18121411_467.jpg
HIF3A-antibody-GTX85702-IHC-2_18121411_667.jpg

Cat No. GTX85702

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Specificity/Sensitivity

aa 581-592 of mouse Hif3 a (Hypoxia Inducible Factor).
PROPERTIES

Form

Liquid

Buffer

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide - KLH conjugated

Purification

This antibody was purified using Immunoaffinity Chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hypoxia Inducible Factor 3, Alpha Subunit,Ipas,Mop7,Nepas,Bhlhe17,Hif3A

Database

Research Area

DATA IMAGES
HIF3A-antibody-GTX85702-IHC-1_18121411_666.jpg

GTX85702 IHC-P Image

Human Lung, Respiratory Epithelium (formalin-fixed, paraffin-embedded) stained with HIF3A antibody (GTX85702) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.

HIF3A-antibody_WB_GTX85702-2_18121411_467.jpg

GTX85702 WB Image

Anti-Hif Antibody - Western Blot. Western blot of Affinity Purified anti-Hif antibody shows detection of a band ~72 kD corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37? C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:1600. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

HIF3A-antibody-GTX85702-IHC-2_18121411_667.jpg

GTX85702 IHC-P Image

Human Placenta (formalin-fixed, paraffin-embedded) stained with HIF3A antibody (GTX85702) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.