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HINT1 antibody

Anti-HINT1 antibody used in Western Blot (WB). GTX64971
Anti-HINT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64971
Anti-HINT1 antibody used in Western Blot (WB). GTX64971
Anti-HINT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64971

Cat. No. GTX64971

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HINT1 (NP_005331.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histidine triad nucleotide binding protein 1 , HINT , NMAN , PKCI-1 , PRKCNH1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-HINT1 antibody used in Western Blot (WB). GTX64971

GTX64971 WB Image

WB analysis of various sample lysates using GTX64971 HINT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HINT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64971

GTX64971 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64971 HINT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-HINT1 antibody used in Western Blot (WB). GTX64971

GTX64971 WB Image

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX64971 HINT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HINT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64971

GTX64971 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX64971 HINT1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399