APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Recommended starting dilution is 1:1000. |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT5C6 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ST15 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ST13 Hsp70 interacting protein , AAG2 , FAM10A1 , FAM10A4 , HIP , HOP , HSPABP , HSPABP1 , P48 , PRO0786 , SNC6 , ST13 , suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) , HSC70 Interacting Protein HIP
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that it is a candidate tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]
Database
Research Area
DATA IMAGES
![Anti-HIP antibody [AT5C6] used in Western Blot (WB). GTX57598](/templates/default/js/lightbox/images/loading.gif "Anti-HIP antibody [AT5C6] used in Western Blot (WB). GTX57598")
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GTX57598 WB Image
WB analysis of various samples using GTX57598 HIP antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : SW480 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : LNCap whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : PC-3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57598 ICC/IF Image
ICC/IF analysis of 293T cells using GTX57598 HIP antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57598 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57598 HIP antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57598 FACS Image
FACS analysis of Hep3B cells using GTX57598 HIP antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
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GTX57598 FACS Image
FACS analysis of 293T cells using GTX57598 HIP antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg
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GTX57598 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57598 HIP antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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REFERENCE
There are currently no references for HIP antibody [AT5C6] (GTX57598). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HIP antibody [AT5C6] (GTX57598). Be the first to share your experience with this product.
![Anti-HIP antibody [AT5C6] used in Western Blot (WB). GTX57598](/upload/website/prouct_img/small/GTX57598/GTX57598_20200204_WB_w_23061123_178.webp "Anti-HIP antibody [AT5C6] used in Western Blot (WB). GTX57598")
![Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598](/upload/website/prouct_img/small/GTX57598/GTX57598_20200204_IF1_w_23061123_343.webp "Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598")
![Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598](/upload/website/prouct_img/small/GTX57598/GTX57598_20200204_IF3_w_23061123_842.webp "Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598")
![Anti-HIP antibody [AT5C6] used in Flow cytometry (FACS). GTX57598](/upload/website/prouct_img/small/GTX57598/GTX57598_20200204_FC2_w_23061123_305.webp "Anti-HIP antibody [AT5C6] used in Flow cytometry (FACS). GTX57598")
![Anti-HIP antibody [AT5C6] used in Flow cytometry (FACS). GTX57598](/upload/website/prouct_img/small/GTX57598/GTX57598_20200204_FC1_w_23061123_744.webp "Anti-HIP antibody [AT5C6] used in Flow cytometry (FACS). GTX57598")
![Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598](/upload/website/prouct_img/small/GTX57598/GTX57598_20200204_IF2_w_23061123_218.webp "Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598")
![Anti-HIP antibody [AT5C6] used in Western Blot (WB). GTX57598](/upload/website/prouct_img/normal/GTX57598/GTX57598_20200204_WB_w_23061123_178.webp "Anti-HIP antibody [AT5C6] used in Western Blot (WB). GTX57598")
![Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598](/upload/website/prouct_img/normal/GTX57598/GTX57598_20200204_IF1_w_23061123_343.webp "Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598")
![Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598](/upload/website/prouct_img/normal/GTX57598/GTX57598_20200204_IF3_w_23061123_842.webp "Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598")
![Anti-HIP antibody [AT5C6] used in Flow cytometry (FACS). GTX57598](/upload/website/prouct_img/normal/GTX57598/GTX57598_20200204_FC2_w_23061123_305.webp "Anti-HIP antibody [AT5C6] used in Flow cytometry (FACS). GTX57598")
![Anti-HIP antibody [AT5C6] used in Flow cytometry (FACS). GTX57598](/upload/website/prouct_img/normal/GTX57598/GTX57598_20200204_FC1_w_23061123_744.webp "Anti-HIP antibody [AT5C6] used in Flow cytometry (FACS). GTX57598")
![Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598](/upload/website/prouct_img/normal/GTX57598/GTX57598_20200204_IF2_w_23061123_218.webp "Anti-HIP antibody [AT5C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57598")