APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.56 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HIP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886489
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
huntingtin interacting protein 1 , HIP-I , ILWEQ , SHON , SHONbeta , SHONgamma
Cellular Localization
Cytoplasm , Nucleus , Endomembrane system , Cytoplasmic vesicle , clathrin-coated vesicle membrane
Background
The product of this gene is a membrane-associated protein that colocalizes with huntingtin. This protein has similarities to cytoskeleton proteins and its interaction with huntingtin is thought to play a functional role in the cell filament network. Loss of normal huntingtin-HIP1 interaction in Huntington disease may contribute to a defect in membrane-cytoskeletal integrity in the brain. This gene could help in the understanding of the normal function of huntingtin and also the pathogenesis of Huntington disease. It also has been implicated in the pathogenesis of hematopoietic malignancies. An alternative splice variant of this gene has been described but its full length sequence has not been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX131449 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIP1 antibody (GTX131449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX131449 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIP1 antibody (GTX131449) diluted at 1:1000.
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REFERENCE
There are currently no references for HIP1 antibody (GTX131449). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HIP1 antibody (GTX131449). Be the first to share your experience with this product.
