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HIP1 antibody

Anti-HIP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32653
Anti-HIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32653
Anti-HIP1 antibody used in Western Blot (WB). GTX32653

Cat. No. GTX32653

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all HIP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 410-690 of human HIP1 (NP_005329.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

huntingtin interacting protein 1 , HIP-I , ILWEQ , SHON , SHONbeta , SHONgamma

Cellular Localization

Cytoplasm,Nucleus

Background

The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-HIP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32653

GTX32653 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX32653 HIP1 antibody.
Blue : DAPI

Anti-HIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32653

GTX32653 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32653 HIP1 antibody.
Dilution : 1:100

Anti-HIP1 antibody used in Western Blot (WB). GTX32653

GTX32653 WB Image

WB analysis of various sample lysates using GTX32653 HIP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399