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HIP1R antibody [N1N2], N-term

Anti-HIP1R antibody [N1N2], N-term used in Western Blot (WB). GTX117072
Anti-HIP1R antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117072
Anti-HIP1R antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117072
Anti-HIP1R antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117072

Cat. No. GTX117072

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all HIP1R products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rabbit, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIP1R. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177621

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

huntingtin interacting protein 1 related , HIP12 , HIP3 , ILWEQ

Cellular Localization

Cytoplasm , perinuclear region , Endomembrane system , Cytoplasmic vesicle , clathrin-coated vesicle membrane

Database

Research Area

DATA IMAGES

Anti-HIP1R antibody [N1N2], N-term used in Western Blot (WB). GTX117072

GTX117072 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX117072 diluted at 1:1000

Anti-HIP1R antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117072

GTX117072 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HIP1 Related(GTX117072) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HIP1R antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117072

GTX117072 ICC/IF Image

HIP1 Related antibody [N1N2], N-term detects HIP1R protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIP1R protein stained by HIP1 Related antibody [N1N2], N-term (GTX117072) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-HIP1R antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117072

GTX117072 IHC-P Image

HIP1 Related antibody [N1N2], N-term detects HIP1 Related protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HIP1 Related antibody [N1N2], N-term (GTX117072) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169