GeneTex
United States (US)

HIP55 antibody [N1], N-term

GTX106288_39701_IHC_19011506_114.jpg
GTX106288_39701_IFA_19011506_675.jpg
GTX106288_WB_19011506_690.jpg

Cat No. GTX106288

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIP55. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ABP1 antibody, HIP-55 antibody, SH3P7 antibody, DBNL antibody, SH3 domain-containing protein 7 antibody, drebrin-like protein antibody, cervical SH3P7 antibody, cervical mucin-associated protein antibody, src homology 3 domain-containing protein HIP-55 antibody, drebrin-F antibody, HPK1-interacting protein of 55 kDa antibody, drebrin-like antibody

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection , lamellipodium

Background

Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.

Database

Research Area

DATA IMAGES
GTX106288_39701_IHC_19011506_114.jpg

GTX106288 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HIP55(GTX106288) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106288_39701_IFA_19011506_675.jpg

GTX106288 ICC/IF Image

HIP55 antibody [N1], N-term detects DBNL protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DBNL protein stained by HIP55 antibody [N1], N-term (GTX106288) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX106288_WB_19011506_690.jpg

GTX106288 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4(GTX27912)
10% SDS PAGE
GTX106288 diluted at 1:1500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169