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HIP55 antibody [N1], N-term

Anti-HIP55 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX106288
Anti-HIP55 antibody [N1], N-term used in Western Blot (WB). GTX106288
Anti-HIP55 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106288

Cat. No. GTX106288

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HIP55 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIP55. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240945

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

drebrin like , ABP1 , HIP-55 , HIP55 , SH3P7

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection , lamellipodium

Background

Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.

Database

Research Area

DATA IMAGES

Anti-HIP55 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX106288

GTX106288 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HIP55(GTX106288) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HIP55 antibody [N1], N-term used in Western Blot (WB). GTX106288

GTX106288 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4(GTX27912)
10% SDS PAGE
GTX106288 diluted at 1:1500

Anti-HIP55 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106288

GTX106288 ICC/IF Image

HIP55 antibody [N1], N-term detects DBNL protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DBNL protein stained by HIP55 antibody [N1], N-term (GTX106288) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169