APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
0.2-2.5 ug/ml |
2-10 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , Jurkat
PROPERTIES
Form
Liquid
Buffer
1x PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to an Internal region of Human HIPK2
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
homeodomain interacting protein kinase 2 , PRO0593
Cellular Localization
Cytoplasm
Background
This gene encodes a conserved serine/threonine kinase that is a member of the homeodomain-interacting protein kinase family. The encoded protein interacts with homeodomain transcription factors and many other transcription factors such as p53, and can function as both a corepressor and a coactivator depending on the transcription factor and its subcellular localization. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Database
Research Area
DATA IMAGES
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GTX30803 WB Image
WB analysis of Jurkat cells using GTX30803 HIPK2 antibody at 0.1μg/ml.
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GTX30803 IHC-P Image
IHC-P analysis of human heart tissue using GTX30803 HIPK2 antibody at 4.0-8.0μg/ml.
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GTX30803 IHC-P Image
IHC-P analysis of human heart tissue using GTX30803 HIPK2 antibody at 1:600. Left to right:DAPI, HIPK2, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.
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GTX30803 IHC-P Image
IHC-P analysis of human pineal tissue using GTX30803 HIPK2 antibody at 1:100. Left to right:DAPI, HIPK2, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.
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GTX30803 WB Image
WB analysis of HepG2 cells using GTX30803 HIPK2 antibody at 1.0μg/ml.
Lane A : Primary Antibody
Lane B : Primary Antibody + Blocking peptide
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GTX30803 IHC-P Image
IHC-P analysis of human brain tissue using GTX30803 HIPK2 antibody at 4.0-8.0μg/ml.
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GTX30803 IHC-P Image
IHC-P analysis of human heart tissue using GTX30803 HIPK2 antibody at 4.0-8.0μg/ml.
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REFERENCE
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REVIEW
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