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HIPPI antibody [4F6]

Overexpression
Anti-HIPPI antibody [4F6] used in Western Blot (WB). GTX84310
Anti-HIPPI antibody [4F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84310
Anti-HIPPI antibody [4F6] used in IHC (Paraffin sections) (IHC-P). GTX84310
Anti-HIPPI antibody [4F6] used in IHC (Paraffin sections) (IHC-P). GTX84310
Anti-HIPPI antibody [4F6] used in IHC (Paraffin sections) (IHC-P). GTX84310
Anti-HIPPI antibody [4F6] used in IHC (Paraffin sections) (IHC-P). GTX84310
Anti-HIPPI antibody [4F6] used in Western Blot (WB). GTX84310

Cat. No. GTX84310

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($519)
See all HIPPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human IFT57(NP_060480) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intraflagellar transport 57 , ESRRBL1 , HIPPI , MHS4R2 , OFD18

Background

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator | however the relevance of such function remains unclear.

Database

Research Area

DATA IMAGES

Anti-HIPPI antibody [4F6] used in Western Blot (WB). GTX84310

GTX84310 WB Image

WB analysis of various cell lines using GTX84310 HIPPI antibody [4F6].
Loading : 35 ug per lane
Dilution : 1:200

Anti-HIPPI antibody [4F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84310

GTX84310 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with HIPPI plasmid using GTX84310 HIPPI antibody [4F6].

Anti-HIPPI antibody [4F6] used in IHC (Paraffin sections) (IHC-P). GTX84310

GTX84310 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84310 HIPPI antibody [4F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-HIPPI antibody [4F6] used in IHC (Paraffin sections) (IHC-P). GTX84310

GTX84310 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84310 HIPPI antibody [4F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-HIPPI antibody [4F6] used in IHC (Paraffin sections) (IHC-P). GTX84310

GTX84310 IHC-P Image

IHC-P analysis of breast tissue using GTX84310 HIPPI antibody [4F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-HIPPI antibody [4F6] used in IHC (Paraffin sections) (IHC-P). GTX84310

GTX84310 IHC-P Image

IHC-P analysis of kidney tissue using GTX84310 HIPPI antibody [4F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-HIPPI antibody [4F6] used in Western Blot (WB). GTX84310

GTX84310 WB Image

Overexpression

WB analysis of HEK293T cells transfected with HIPPI plasmid (Right) or empty vector (Left) for 48 hrs using GTX84310 HIPPI antibody [4F6].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519