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HIPPI antibody [C1C3]

Anti-HIPPI antibody [C1C3] used in Western Blot (WB). GTX111125
Anti-HIPPI antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111125
Anti-HIPPI antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111125

Cat. No. GTX111125

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HIPPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIPPI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950553

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intraflagellar transport 57 , ESRRBL1 , HIPPI , MHS4R2 , OFD18

Cellular Localization

Cell projection , cilium basal body

Background

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

Database

Research Area

DATA IMAGES

Anti-HIPPI antibody [C1C3] used in Western Blot (WB). GTX111125

GTX111125 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX111125 diluted at 1:1000

Anti-HIPPI antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111125

GTX111125 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HIPPI(GTX111125) antibody at 1:200 dilution.

Anti-HIPPI antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111125

GTX111125 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using HIPPI(GTX111125) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169