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HIRIP3 antibody

Anti-HIRIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65853
Anti-HIRIP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65853
Anti-HIRIP3 antibody used in Western Blot (WB). GTX65853
Anti-HIRIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65853
Anti-HIRIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65853

Cat. No. GTX65853

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HIRIP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HIRA interacting protein 3

Cellular Localization

Nucleus

Background

The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-HIRIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65853

GTX65853 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX65853 HIRIP3 antibody.
Dilution : 1:100

Anti-HIRIP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65853

GTX65853 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX65853 HIRIP3 antibody.

Anti-HIRIP3 antibody used in Western Blot (WB). GTX65853

GTX65853 WB Image

WB analysis of various sample lysates using GTX65853 HIRIP3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HIRIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65853

GTX65853 IHC-P Image

IHC-P analysis of human well-differentiated squamous skin carcinoma tissue using GTX65853 HIRIP3 antibody.
Dilution : 1:100

Anti-HIRIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65853

GTX65853 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX65853 HIRIP3 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399