APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant funsion protein containing a sequence corresponding to amino acids 1-126 of human HIST2H2BE (NP_003519.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
histone cluster 2 H2B family member e , GL105 , H2B , H2B.1 , H2BFQ , H2BGL105 , H2BQ
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. [provided by RefSeq, Aug 2015]
Database
Research Area
DATA IMAGES
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GTX65940 WB Image
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX65940 HIST2H2BE antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX65940 WB Image
WB analysis of various sample lysates using GTX65940 HIST2H2BE antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX65940 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX65940 HIST2H2BE antibody.
Dilution : 1:100
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GTX65940 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX65940 HIST2H2BE antibody.
Dilution : 1:100
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GTX65940 IHC-P Image
IHC-P analysis of human esophagus tissue using GTX65940 HIST2H2BE antibody.
Dilution : 1:100
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REFERENCE
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REVIEW
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