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HIV protease antibody [1696]

Anti-HIV protease antibody [1696] used in Dot blot (Dot). GTX28327

Cat No. GTX28327

Host Mouse
Clonality Monoclonal
Clone Name 1696
Isotype IgG1
Application WB, Dot, ELISA, Inhibition assay
Reactivity Human Immunodeficiency virus
Package
100μg ($319)
APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For Inhib: Use at an assay dependent dilution. Inhibits the enzyme activity of HIV-1 and HIV-2 proteases. For WB (reducing conditions): Use at a concentration of 0 . 5 μ g / m l. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

Recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes viral polyproteins into functional proteins
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human Immunodeficiency virus

Immunogen

Recombinant HIV-1 protease.

Purification

Protein A affinity purified
> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-HIV protease antibody [1696] used in Dot blot (Dot). GTX28327

GTX28327 Dot Image

Dot Blot analysis of recombinant HIV protease. The total amount of recombinant HIV-protease spotted on the nitrocellulose membrane are indicated in left column.
Lane 1: anti-HIV protease (1696) (GTX28327); 0.2 μg/ml
Lane 2: anti-HIV protease (1696) (GTX28327); 1.0 μg/ml
Lane 3: anti-HIV protease (1696) (GTX28327); 2.0 μg/ml

Package List Price ($)
$ 319