For ELISA: Use at an assay dependent dilution. For Inhib: Use at an assay dependent dilution. Inhibits the enzyme activity of HIV-1 and HIV-2 proteases. For WB (reducing conditions): Use at a concentration of 0 . 5 μ g / m l. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Optimal dilutions/concentrations should be determined by the researcher.
Recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes viral polyproteins into functional proteins
Phosphate-buffered saline, pH 7.4 containing 15mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Human Immunodeficiency virus
Recombinant HIV-1 protease.
Protein A affinity purified
> 95% (by SDS-PAGE)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.