GeneTex
  • Country / Location Selection

United States (US)

HIV protease antibody [1696]

Anti-HIV protease antibody [1696] used in Dot blot (Dot). GTX28327

Cat No. GTX28327

Host Mouse
Clonality Monoclonal
Clone Name 1696
Isotype IgG1
Application WB, Dot, ELISA, Inhibition assay
Reactivity Human Immunodeficiency virus
Package
100 μg ($319)
APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For Inhib: Use at an assay dependent dilution. Inhibits the enzyme activity of HIV-1 and HIV-2 proteases. For WB (reducing conditions): Use at a concentration of 0 . 5 μ g / m l. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

Recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes viral polyproteins into functional proteins
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human Immunodeficiency virus

Immunogen

Recombinant HIV-1 protease.

Purification

Protein A affinity purified
> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-HIV protease antibody [1696] used in Dot blot (Dot). GTX28327

GTX28327 Dot Image

Dot Blot analysis of recombinant HIV protease. The total amount of recombinant HIV-protease spotted on the nitrocellulose membrane are indicated in left column.
Lane 1: anti-HIV protease (1696) (GTX28327); 0.2 μg/ml
Lane 2: anti-HIV protease (1696) (GTX28327); 1.0 μg/ml
Lane 3: anti-HIV protease (1696) (GTX28327); 2.0 μg/ml

REFERENCE
There are currently no references for HIV protease antibody [1696] (GTX28327). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HIV protease antibody [1696] (GTX28327). Be the first to share your experience with this product.