Detection of HIV-1 proteinase in the extract of HIV-1 infected cells by Western blotting using anti-HIV-1 protease antibody. 1: Purified HIV-1 protease (1 ng) 2: Extract of MT4 cells 3.: Extract of MT4 cells infected with HIV-1 (LAI strain) The antiserum was used at 1/2,000 dilution.

Dot blotting of HIV-1 protease by using anti-HIV-1 protease antibody Anti-HIV-1 protease antibody was used at 1/2,000 dilution. As second antibody, goat anti-rabbit IgG antibody conjugated with HRP was used at 1/5,000 dilution. ECL system was used.

ELISA of HIV-1 protease with anti-HIV-1 protease antibody The antibody was used at dilutions indicated above. Purified Protease was spotted on wells.