GeneTex
United States (US)

HIV1 protease antibody

GTX64132_20170328_ELISA_18121411_823.jpg
GTX64132_20170328_Dot_18121411_167.jpg
GTX64132_20170328_WB_18121411_412.jpg

Cat No. GTX64132

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, Dot, ELISA, Inhibition assay
Reactivity Human Immunodeficiency virus
APPLICATION

Application Note

Western blotting (1:2,000), Dot blotting (1:2,000), Immunoprecipitation (1:200), ELISA. (1:10,000)
PROPERTIES

Form

Liquid

Buffer

Whole antiserum added with0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Full-size functional recombinant HIV-1 protease expressed and purified from E. coli

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX64132_20170328_ELISA_18121411_823.jpg

GTX64132 ELISA Image

ELISA of HIV-1 protease with anti-HIV-1 protease antibody The antibody was used at dilutions indicated above. Purified Protease was spotted on wells.

GTX64132_20170328_Dot_18121411_167.jpg

GTX64132 Dot Image

Dot blotting of HIV-1 protease by using anti-HIV-1 protease antibody Anti-HIV-1 protease antibody was used at 1/2,000 dilution. As second antibody, goat anti-rabbit IgG antibody conjugated with HRP was used at 1/5,000 dilution. ECL system was used.

GTX64132_20170328_WB_18121411_412.jpg

GTX64132 WB Image

Detection of HIV-1 proteinase in the extract of HIV-1 infected cells by Western blotting using anti-HIV-1 protease antibody. 1: Purified HIV-1 protease (1 ng) 2: Extract of MT4 cells 3.: Extract of MT4 cells infected with HIV-1 (LAI strain) The antiserum was used at 1/2,000 dilution.

Package List Price ($)
$ 349