GeneTex
United States (US)

HL-60 whole cell lysate

Cat No. GTX27914

Application WB
Species Human
PRODUCT

Product Description

HL-60 lysate was prepared by homogenization in modified RIPA buffer. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The Jurkat lysate was boiled for 5 min in 1 x SDS sample buffer containing 5% β-mercaptoethanol.
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

1 x SDS sample buffer (50 mM Tris-HCl (pH 6.8), 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2mg/ml

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Package List Price ($)
$ 179