APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human HLA-A (NP_001229687.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
major histocompatibility complex, class I, A , HLAA
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX54099 WB Image
WB analysis of various sample lysates using GTX54099 HLA-A antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54099 IHC-P Image
IHC-P analysis of human esophagus tissue using GTX54099 HLA-A antibody.
Dilution : 1:100
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GTX54099 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX54099 HLA-A antibody.
Dilution : 1:100
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GTX54099 IHC-P Image
IHC-P analysis of human liver damage tissue using GTX54099 HLA-A antibody.
Dilution : 1:100
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REFERENCE
There are currently no references for HLA-A antibody (GTX54099). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HLA-A antibody (GTX54099). Be the first to share your experience with this product.
