Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| 10μg/ml |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Neutralizing/Inhibition
Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.
|
Not tested in other applications.
Calculated MW
Product Note
The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity..
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Papain solubilised HLA-A2
Purification
Protein A purified
From tissue culture supernatant
Endotoxin
< 0.01 EU/μg (determined by the LAL assay)
Conjugation
Unconjugated
RRID
AB_377044
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
Major Histocompatibility Complex, Class I, A , Hlaa , Hla-A
Background
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
Research Area
![Anti-HLA-A2 antibody [BB7.2] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX75806](/upload/website/prouct_img/small/GTX75806/GTX75806_3793_FACS_w_23061322_961.webp "Anti-HLA-A2 antibody [BB7.2] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX75806")
![Anti-HLA-A2 antibody [BB7.2] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX75806](/upload/website/prouct_img/normal/GTX75806/GTX75806_3793_FACS_w_23061322_961.webp "Anti-HLA-A2 antibody [BB7.2] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX75806")