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HLA-A2 antibody [BB7.2] (Low endotoxin, azide free)

Anti-HLA-A2 antibody [BB7.2] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX75806

Cat. No. GTX75806

Host

Mouse

Clonality

Monoclonal

Clone Name

BB7.2

Isotype

IgG2b

Application

ICC/IF, IHC-Fr, FACS, IP, Blocking, Neutralizing/Inhibition

Reactivity

Human
Package
500 μg ($739)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 10μg/ml
IP Assay dependent
Blocking Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Neutralizing/Inhibition
Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity..

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Papain solubilised HLA-A2

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377044

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Major Histocompatibility Complex, Class I, A , Hlaa , Hla-A

Background

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES

Anti-HLA-A2 antibody [BB7.2] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX75806

GTX75806 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX75806 HLA-A2 antibody [BB7.2] (Low endotoxin, azide free).

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 739
Custom Conjugations