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HLA-A2 antibody [BB7.2] (PE)

Cat. No. GTX80221

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BB7.2

Isotype

 IgG2b

Application

 FACS

Reactivity

 Human
Package
100 test ($329)
See all HLA-A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody recognizes an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands within the human HLA-A2 histocompatibility antigen.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HLA-A2 solubilised by papain

Purification

Purified by size-exclusion chromatography

Conjugation

Phycoerythrin (PE) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HLAA , major histocompatibility complex, class I, A

Cellular Localization

Membrane

Background

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Clone Reference

David Rep?raz et al. J Immunother Cancer 2022; 10 (2) Neoantigens as potential vaccines in hepatocellular carcinoma.
Bonaventura P et al. Sci Adv 2022; 8 (4):eabj3671 Identification of shared tumor epitopes from endogenous retroviruses inducing high-avidity cytotoxic T cells for cancer immunotherapy.
Wu L et al. Proc Natl Acad Sci U S A 2018; 115 (19):E4453-E4462 Induction of antitumor cytotoxic lymphocytes using engineered human primary blood dendritic cells.
Liu B et al. Eur J Immunol 2014; 44 (1):239-50 2D TCR-pMHC-CD8 kinetics determines T-cell responses in a self-antigen-specific TCR system.
Chung B et al. Cell Rep 2014; 8 (3):871-82 Antigen-Specific Inhibition of High-Avidity T Cell Target Lysis by Low-Avidity T Cells via Trogocytosis.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329