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HLA-ABC antibody [YTH 862.2]

Anti-HLA-ABC antibody [YTH 862.2] used in Flow cytometry (FACS). GTX76213

Cat No. GTX76213

Host

Rat

Clonality

Monoclonal

Clone Name

YTH 862.2

Isotype

IgG2b

Application

ICC/IF, IHC-Fr, FACS, IP, Activation

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1/50-1/100
IP Assay dependent
Activation Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Activation
Rat anti Human HLA ABC antibody, clone YTH862.2 may be able to induce apoptosis in activated but not resting T cells (Genestier et al. 1997).

Not tested in other applications.

Specificity/Sensitivity

This antibody recognizes a monomorphic determinant on the human HLA Class I heavy chain.Epitope analysis has shown that this antibody binds in the alpha1 domain. Clone YTH862.2 may be able to induce apoptosis in activated but not resting T cells (Genestier et al. 1997).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PHA activated human peripheral blood lymphocytes.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-HLA-ABC antibody [YTH 862.2] used in Flow cytometry (FACS). GTX76213

GTX76213 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX76213 HLA-ABC antibody [YTH 862.2].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319