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HLA-B antibody, N-term

Anti-HLA-B antibody, N-term used in Flow cytometry (FACS). GTX81138
Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138
Anti-HLA-B antibody, N-term used in Flow cytometry (FACS). GTX81138
Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138
Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138
Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138
Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138

Cat. No. GTX81138

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
400 μl ($399)
See all HLA-B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
FACS 1:10-1:50
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class I, B , AS , B-4901 , HLAB

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-B antibody, N-term used in Flow cytometry (FACS). GTX81138

GTX81138 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX81138 HLA-B antibody, N-term.
Green : HLA-B
Blue : Isotype control
Dilution : 1:25

Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138

GTX81138 WB Image

WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HLA-B (Lane 2) using GTX81138 HLA-B antibody, N-term.

Anti-HLA-B antibody, N-term used in Flow cytometry (FACS). GTX81138

GTX81138 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX81138 HLA-B antibody, N-term.
Green : HLA-B
Blue : Isotype control
Dilution : 1:25

Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138

GTX81138 WB Image

WB analysis of HL-60, Jurkat, Ramos cell using GTX81138 HLA-B antibody, N-term.
Loading : 35ug per lane
Dilution : 1:1000

Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138

GTX81138 WB Image

WB analysis of A375 cell lysate (35ug/lane) using GTX81138 HLA-B antibody, N-term.

Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138

GTX81138 WB Image

WB analysis of CEM, HL-60, Jurkat, Ramos cell using GTX81138 HLA-B antibody, N-term.
Loading : 35ug per lane
Dilution : 1:1000

Anti-HLA-B antibody, N-term used in Western Blot (WB). GTX81138

GTX81138 WB Image

WB analysis of A2058 cell lysate (35ug/lane) using GTX81138 HLA-B antibody, N-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399