GeneTex

  • Country / Location Selection

United States (US)

HLA-B27 antibody [HLA-ABC-m3] (FITC)

Cat. No. GTX74708

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HLA-ABC-m3

Isotype

 IgG2a

Application

 FACS

Reactivity

 Human
Package
500 μl ($329)
See all HLA-B27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Product Note

This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

50 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.

Purification

Purified by ion exchange chromatography

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_376424

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class I, B , AS , B-4901 , HLAB

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Liu CH et al. J Clin Invest 2019; (Epub) HLA-B27-mediated activation of TNAP phosphatase promotes pathogenic syndesmophyte formation in ankylosing spondylitis.
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for HLA-B27 antibody [HLA-ABC-m3] (FITC) (GTX74708). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329