APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Neat |
|
Note :
FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
|
Not tested in other applications.
Product Note
This antibody recognizes the HLA-B7 antigen and does not cross-react with HLA-B27 or other related antigens.It can be used to distinguish true HLA B27 positives from false HLA B27 positives (i.e. HLA B7 positive) in the investigation of diseases such as ankylosing spondylitis and anterior uveitis.
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 5% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Papain solubilized HLA-B7 antigen
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Phycoerythrin (PE)
RRID
AB_377468
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
major histocompatibility complex, class I, B , AS , B-4901 , HLAB
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for HLA-B7 antibody [BB7.1] (PE) (GTX76657). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HLA-B7 antibody [BB7.1] (PE) (GTX76657). Be the first to share your experience with this product.
