GeneTex
United States (US)

HLA Class I antibody [MEM-147]

Cat No. GTX29090

Host Mouse
Clonality Monoclonal
Clone Name MEM-147
Isotype IgG1
Application WB, FACS, IP
Reactivity Human
APPLICATION

Application Note

FACS: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/100. Use in non reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

Reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (GTX27855).
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

PHA-activated peripheral blood lymphocytes

Purification

>95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Package List Price ($)
$ 319