GeneTex

  • Country / Location Selection

United States (US)

HLA Class I antibody [W6/32] (Low endotoxin, azide free)

Cat. No. GTX76545

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 W6/32

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, ELISA, Functional Assay

Reactivity

 Human, Bovine, Baboon, Chimpanzee, Cynomolgus monkey, Gorilla, Rhesus Monkey, Shrew
Package
100 μg ($399)
See all HLA Class I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1/50-1/100
IP Assay dependent
ELISA Assay dependent
Functional Assay Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Product Note

This antibody recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human tonsil lymphocyte membranes.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_377402

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

REFERENCE

Clone Reference

Sekijima M et al. Xenotransplantation 2019;:e12543 Preparation of hybrid porcine thymus containing non-human primate thymic epithelial cells in miniature swine.
Wumin Li et al. Vet Immunol Immunopathol 1992; 33 (4):309-320 Bovine major histocompatibility complex class I specific monoclonal antibodies characterized by flow cytometry, one- and two-dimensional electrophoresis, Western blot and inhibition of cytotoxic T lymphocyte function
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for HLA Class I antibody [W6/32] (Low endotoxin, azide free) (GTX76545). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations