*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLa , HepG2 , Jurkat , Raji
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human HLA-DMA. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Major Histocompatibility Complex, Class Ii, Dm Alpha,D6S222E,Dma,Hladm,Ring6,Hla-Dma
HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq]
GTX114939 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HLA-DMA(GTX114939) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX114939 WB Image
HLA-DMA antibody detects HLA-DMA protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
12 % SDS-PAGE
HLA-DMA antibody (GTX114939) dilution: 1:500
GTX114939 WB Image
Sample (30 ug of whole cell lysate)
12% SDS PAGE
GTX114939 diluted at 1:1000