GeneTex
United States (US)

HLA-DMA antibody

GTX114939_40240_IHC_18121410_610.jpg
GTX114939_40240_WB_18121410_288.jpg
GTX114939_WB_18121410_976.jpg

Cat No. GTX114939

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HeLa , HepG2 , Jurkat , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DMA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Major Histocompatibility Complex, Class Ii, Dm Alpha,D6S222E,Dma,Hladm,Ring6,Hla-Dma

Background

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq]
DATA IMAGES
GTX114939_40240_IHC_18121410_610.jpg

GTX114939 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using HLA-DMA(GTX114939) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114939_40240_WB_18121410_288.jpg

GTX114939 WB Image

HLA-DMA antibody detects HLA-DMA protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
12 % SDS-PAGE
HLA-DMA antibody (GTX114939) dilution: 1:500

GTX114939_WB_18121410_976.jpg

GTX114939 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
GTX114939 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169