APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HLA-DMB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950494
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
major histocompatibility complex, class II, DM beta , D6S221E , RING7
Cellular Localization
Late endosome membrane , Lysosome membrane
Background
HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-HLA-DMB antibody [C2C3], C-term used in Western Blot (WB). GTX108492](/templates/default/js/lightbox/images/loading.gif "Anti-HLA-DMB antibody [C2C3], C-term used in Western Blot (WB). GTX108492")
|
GTX108492 WB Image
Western Blot analysis of HLA-DMB expression in transfected 293T cell line by HLA-DMB polyclonal antibody.
A:Non-transfected lysate.
B:HLA-DMB transfected lysate.
12% SDS PAGE
GTX108492 diluted at 1:500
|
|
|
GTX108492 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HLA-DMB(GTX108492) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
REFERENCE
There are currently no references for HLA-DMB antibody [C2C3], C-term (GTX108492). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HLA-DMB antibody [C2C3], C-term (GTX108492). Be the first to share your experience with this product.
![Anti-HLA-DMB antibody [C2C3], C-term used in Western Blot (WB). GTX108492](/upload/website/prouct_img/small/GTX108492/GTX108492_WB_19011507_360.jpg "Anti-HLA-DMB antibody [C2C3], C-term used in Western Blot (WB). GTX108492")
![Anti-HLA-DMB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108492](/upload/website/prouct_img/small/GTX108492/GTX108492_39812_IHC_19011507_505.jpg "Anti-HLA-DMB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108492")
![Anti-HLA-DMB antibody [C2C3], C-term used in Western Blot (WB). GTX108492](/upload/website/prouct_img/normal/GTX108492/GTX108492_WB_19011507_360.jpg "Anti-HLA-DMB antibody [C2C3], C-term used in Western Blot (WB). GTX108492")
![Anti-HLA-DMB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108492](/upload/website/prouct_img/normal/GTX108492/GTX108492_39812_IHC_19011507_505.jpg "Anti-HLA-DMB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108492")