GeneTex
United States (US)

HLA-DMB antibody [C2C3], C-term

GTX108492_WB_19011507_360.jpg
GTX108492_39812_IHC_19011507_505.jpg

Cat No. GTX108492

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HLA-DMB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

D6S221E antibody, RING7 antibody, HLA-DMB antibody, really interesting new gene 7 protein antibody, MHC class II HLA-DMB antibody, "HLA class II histocompatibility antigen, DM beta chain antibody", "class II histocompatibility antigen, M beta chain antibody", MHC class II antigen HLA-DM beta chain antibody, MHC class II antigen DMB antibody, "major histocompatibility complex, class II, DM beta antibody"

Cellular Localization

Late endosome membrane , Lysosome membrane

Background

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq]
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Database

DATA IMAGES
GTX108492_WB_19011507_360.jpg

GTX108492 WB Image

Western Blot analysis of HLA-DMB expression in transfected 293T cell line by HLA-DMB polyclonal antibody.
A:Non-transfected lysate.
B:HLA-DMB transfected lysate.
12% SDS PAGE
GTX108492 diluted at 1:500

GTX108492_39812_IHC_19011507_505.jpg

GTX108492 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HLA-DMB(GTX108492) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169