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HLA-DOA antibody

Anti-HLA-DOA antibody used in Western Blot (WB). GTX33991
Anti-HLA-DOA antibody used in IHC (Paraffin sections) (IHC-P). GTX33991

Cat. No. GTX33991

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all HLA-DOA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:100-1:300
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthesized peptide derived from the Internal region of human HLA-DOalpha at 40-120 aa, Internal.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HLADNA,HLADOA,HLADZ,HLADZA,major histocompatibility complex, class II, DO alpha,HLA-DOA

Cellular Localization

Endosome membrane,Lysosome membrane

Background

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-DOA antibody used in Western Blot (WB). GTX33991

GTX33991 WB Image

WB analysis of COLO205 cell lysate using GTX33991 HLA-DOA antibody.

Anti-HLA-DOA antibody used in IHC (Paraffin sections) (IHC-P). GTX33991

GTX33991 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX33991 HLA-DOA antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Tris-EDTA, pH8.0 under high-pressure and temperature
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399