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HLA-DPA1 antibody

Anti-HLA-DPA1 antibody used in Western Blot (WB). GTX104414
Anti-HLA-DPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104414
Anti-HLA-DPA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104414

Cat No. GTX104414

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Raji
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DPA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

major histocompatibility complex, class II, DP alpha 1 , DP(W3) , DP(W4) , DPA1 , HLA-DP1A , HLADP , HLASB , PLT1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HLA-DPA1 antibody used in Western Blot (WB). GTX104414

GTX104414 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX104414 diluted at 1:1000

Anti-HLA-DPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104414

GTX104414 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HLA-DPA1(GTX104414) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HLA-DPA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104414

GTX104414 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HLA-DPA1(GTX104414) antibody at 1:500 dilution.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf