GeneTex
United States (US)

HLA-DPA1 antibody

GTX104414_40772_WB_19011506_516.jpg
GTX104414_40772_IHC_19011506_487.jpg
GTX104414_40772_IFA_19011506_278.jpg

Cat No. GTX104414

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Raji
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DPA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HLA-DP1A antibody, HLADP antibody, HLASB antibody, PLT1 antibody, HLA-DPA1 antibody, "HLA class II histocompatibility antigen, DP alpha chain antibody", MHC class II antigen antibody, MHC class II DP3-alpha antibody, "HLA class II histocompatibility antigen, DP alpha 1 chain antibody", Primed lymphocyte test-1 antibody, MHC class II DPA1 antibody, HLA-SB alpha chain antibody, MHC class II HLA-DPA1 antigen antibody, "major histocompatibility complex, class II, DP alpha 1 antibody"

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq]
Show More +

Database

Research Area

DATA IMAGES
GTX104414_40772_WB_19011506_516.jpg

GTX104414 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX104414 diluted at 1:1000

GTX104414_40772_IHC_19011506_487.jpg

GTX104414 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HLA-DPA1(GTX104414) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104414_40772_IFA_19011506_278.jpg

GTX104414 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HLA-DPA1(GTX104414) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf