GeneTex

  • Country / Location Selection

United States (US)

HLA-DPA1 antibody

Anti-HLA-DPA1 antibody used in Western Blot (WB). GTX104414
Anti-HLA-DPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104414
Anti-HLA-DPA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104414

Cat. No. GTX104414

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HLA-DPA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DPA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173176

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DP alpha 1 , DP(W3) , DP(W4) , DPA1 , HLA-DP1A , HLADP , HLASB , PLT1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HLA-DPA1 antibody used in Western Blot (WB). GTX104414

GTX104414 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HLA-DPA1 antibody (GTX104414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HLA-DPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104414

GTX104414 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HLA-DPA1(GTX104414) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HLA-DPA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104414

GTX104414 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HLA-DPA1(GTX104414) antibody at 1:500 dilution.

REFERENCE

Application Reference

Feng Li et al. Animal Model Exp Med 2021; 4 (2) : 116-128 Generation and expression analysis of BAC humanized mice carrying HLA-DP401 haplotype.
Application : IHC-Fr
Reactivity : Mouse
Li F et al. Animal Models and Experimental Medicine 2021; (Epub) Generation and expression analysis of BAC humanized mice carrying HLA-DP401 haplotype.
Submit a Reference

REVIEW

There are currently no reviews for HLA-DPA1 antibody (GTX104414). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169