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HLA-DQA1 antibody [GT1231]

Recombinant antibody
Anti-HLA-DQA1 antibody [GT1231] used in Western Blot (WB). GTX02828
Anti-HLA-DQA1 antibody [GT1231] used in Western Blot (WB). GTX02828
Anti-HLA-DQA1 antibody [GT1231] used in IHC (Paraffin sections) (IHC-P). GTX02828
Anti-HLA-DQA1 antibody [GT1231] used in IHC (Paraffin sections) (IHC-P). GTX02828
Anti-HLA-DQA1 antibody [GT1231] used in IHC (Paraffin sections) (IHC-P). GTX02828

Cat. No. GTX02828

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1231

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all HLA-DQA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human HLA-DQA1

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DQ alpha 1 , CELIAC1 , DQ-A1 , DQA1 , HLA-DQA

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-DQA1 antibody [GT1231] used in Western Blot (WB). GTX02828

GTX02828 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DQA1 antibody [GT1231] (GTX02828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HLA-DQA1 antibody [GT1231] used in Western Blot (WB). GTX02828

GTX02828 WB Image

WB analysis of various samples using GTX02828 HLA-DQA1 antibody [GT1231].
Dilution : 1:1000
Loading : 25μg

Anti-HLA-DQA1 antibody [GT1231] used in IHC (Paraffin sections) (IHC-P). GTX02828

GTX02828 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX02828 HLA-DQA1 antibody [GT1231].
Dilution : 1:100

Anti-HLA-DQA1 antibody [GT1231] used in IHC (Paraffin sections) (IHC-P). GTX02828

GTX02828 IHC-P Image

IHC-P analysis of mouse spleen tissue section using GTX02828 HLA-DQA1 antibody [GT1231].
Dilution : 1:100

Anti-HLA-DQA1 antibody [GT1231] used in IHC (Paraffin sections) (IHC-P). GTX02828

GTX02828 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX02828 HLA-DQA1 antibody [GT1231].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 419