GeneTex
United States (US)

HLA-DQA1 antibody

GTX32654_20180530_IHC-P_1_18121410_635.jpg
GTX32654_20180530_WB_18121410_393.jpg
GTX32654_20180530_IHC-P_18121410_629.jpg

Cat No. GTX32654

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

28 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-216 of human HLA-DQA1 (NP_002113.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Major Histocompatibility Complex, Class Ii, Dq Alpha 1,Celiac1,Dq-A1,Hla-Dqa,Hla-Dqa1

Background

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32654_20180530_IHC-P_1_18121410_635.jpg

GTX32654 IHC-P Image

IHC-P analysis of human prostate tissue using GTX32654 HLA-DQA1 antibody.
Dilution : 1:100

GTX32654_20180530_WB_18121410_393.jpg

GTX32654 WB Image

WB analysis of various samples using GTX32654 HLA-DQA1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32654_20180530_IHC-P_18121410_629.jpg

GTX32654 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32654 HLA-DQA1 antibody.
Dilution : 1:100

Package List Price ($)
$ 329