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HLA-DR antibody [L243] (APC)

Anti-HLA-DR antibody [L243] (APC) used in Flow cytometry (FACS). GTX00505-07

Cat. No. GTX00505-07

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 L243

Isotype

 IgG2a

Application

 FACS

Reactivity

 Human
Package
100 test ($359)
See all HLA-DR products
PRODUCT

Summary

The L243 antibody reacts with a member of the human MHC Class II antigens, HLA-DR. The HLA-DR antigen is expressed on B lymphocytes, activated T lymphocytes, activated NK cells, monocytes, macrophages, other antigen presenting cells and progenitor cells. The L243 antibody is specific to an epitope on the alpha subunit of the heterodimeric HLA-DR protein and binds a different epitope than the LN3 antibody clone. It does not cross-react with HLA-DP or HLA-DQ. This antibody is reported to be cross-reactive with non-human primates including Chimpanzee, Cynomolgus, Rhesus and Baboon. Please choose the appropriate format for each application.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Product Note

This antibody recognizes specifically HLA-DR molecules, both peptide-loaded and empty.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human B lymphocytes

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-HLA-DR antibody [L243] (APC) used in Flow cytometry (FACS). GTX00505-07

GTX00505-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX00505-07 HLA-DR antibody [L243] (APC).
Solid line : Primary antibody
Dashed line : APC mouse IgG2a isotype control
Antibody amount : 0.25 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 359