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HLA-DR antibody [N2C3]

Anti-HLA-DR  antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113459
Anti-HLA-DR  antibody [N2C3] used in Western Blot (WB). GTX113459

Cat No. GTX113459

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , Molt-4 , Raji

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DRB1 antibody, DRw10 antibody, FLJ75017 antibody, FLJ76359 antibody, HLA-DR1B antibody, HLA-DRB antibody, HLA-DRB1* antibody, SS1 antibody, HLA-DRB1 antibody, lymphocyte antigen DRB1 antibody, human leucocyte antigen DRB1 antibody, MHC class II antigen antibody, "major histocompatibility complex, class II, DR beta 1 antibody", "HLA class II histocompatibility antigen, DR-1 beta chain antibody", DW2.2/DR2.2 antibody, MHC class II HLA-DRw10-beta antibody, MHC class II HLA-DR-beta cell surface glycoprotein antibody, MHC class II HLA-DR beta 1 chain antibody

Cellular Localization

Membrane

Background

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq]

Research Area

DATA IMAGES
Anti-HLA-DR  antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113459

GTX113459 IHC-P Image

HLA-DR antibody [N2C3] detects HLA-DR protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
HLA-DR antibody [N2C3] (GTX113459) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HLA-DR  antibody [N2C3] used in Western Blot (WB). GTX113459

GTX113459 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX113459 diluted at 1:1000

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169