Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DR antibody [N2C3] (GTX113459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

HLA-DR antibody [N2C3] detects HLA-DR protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
HLA-DR antibody [N2C3] (GTX113459) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min