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HLA-DR antibody [N2C3]

Anti-HLA-DR antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113459
Anti-HLA-DR antibody [N2C3] used in Western Blot (WB). GTX113459

Cat. No. GTX113459

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HLA-DR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

Raji

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037427

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DR alpha , major histocompatibility complex, class II, DR beta 1 , major histocompatibility complex, class II, DR beta 3 , major histocompatibility complex, class II, DR beta 4 , major histocompatibility complex, class II, DR beta 5 , HLA-DRA1 , DRB1 , HLA-DR1B , HLA-DRB , SS1 , DRB3 , HLA-DPB1 , HLA-DR1B , HLA-DR3B , DR4 , DRB4 , HLA-DR4B

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome membrane,Lysosome membrane,Cell membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-HLA-DR antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113459

GTX113459 IHC-P Image

HLA-DR antibody [N2C3] detects HLA-DR protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
HLA-DR antibody [N2C3] (GTX113459) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HLA-DR antibody [N2C3] used in Western Blot (WB). GTX113459

GTX113459 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DR antibody [N2C3] (GTX113459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Yamagiwa S et al. Int J Med Sci 2014; 11 (9) : 850-6 Presence of antibodies against self human leukocyte antigen class II molecules in autoimmune hepatitis.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169