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HLA-DR beta antibody [N1C3]

Anti-HLA-DR beta antibody [N1C3] used in Western Blot (WB). GTX102047

Cat No. GTX102047

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA DR beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DR-13 antibody, DR-14 antibody, DR13 antibody, DR14 antibody, DRB4 antibody, HLA-DR4B antibody, HLA-DRB4 antibody, leukocyte antigen antibody, human leucocyte antigen DRB4 antibody, MHC HLA DR-beta chain antibody, "major histocompatibility complex, class II, DR beta 4 antibody", MHC class2 antigen antibody, "HLA class II histocompatibility antigen, DR beta 4 chain antibody", MHC class II antigen DRB4 antibody, DR-4 antibody, MHC class II antigen HLA-DR-beta antibody, DR4 antibody, DRB1 transplantation antigen antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq]

Research Area

DATA IMAGES
Anti-HLA-DR beta antibody [N1C3] used in Western Blot (WB). GTX102047

GTX102047 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX102047 diluted at 1:1000

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169