HLA-DRA antibody [N2C3]

Name: HLA-DRA antibody [N2C3]
Brand: GeneTex
SKU: GTX113732
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-HLA-DRA antibody [N2C3] used in Western Blot (WB). GTX113732

Anti-HLA-DRA antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113732

Anti-HLA-DRA antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113732

Cat. No. GTX113732

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all HLA-DRA products

View product citations for antibody GTX113732 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DRA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950495

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DR alpha , HLA-DRA1

Cellular Localization

Membrane

Background

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq]

Database

Gene ID: 3122 HLA-DRA
UniProt: P01903 HLA-DRA

Research Area

DATA IMAGES

GTX113732 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DRA antibody [N2C3] (GTX113732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 24045666

GTX113732 IHC-P Image

HLA-DRA antibody [N2C3] detects HLA-DRA protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HLA-DRA stained by HLA-DRA antibody [N2C3] (GTX113732) diluted at 1:10000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113732 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-231 xenograft, using HLA-DRA(GTX113732) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113732 ICC/IF Image

HLA-DRA antibody [N2C3] detects HLA-DRA protein by immunofluorescent analysis.
Sample: Raji cells were fixed in 100% Methenol for 5 min.
Green: HLA-DRA protein stained by HLA-DRA antibody [N2C3] (GTX113732) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Sreevalsan S et al. EMBO Rep 2020; 21 (12) : e50155 MLLT6 maintains PD-L1 expression and mediates tumor immune resistance.

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REVIEW