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HLA-DRA antibody [SC06-78]

Recombinant antibody
Anti-HLA-DRA antibody [SC06-78] used in Western Blot (WB). GTX01049
Anti-HLA-DRA antibody [SC06-78] used in Western Blot (WB). GTX01049
Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049
Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049
Anti-HLA-DRA antibody [SC06-78] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01049
Anti-HLA-DRA antibody [SC06-78] used in Flow cytometry (FACS). GTX01049
Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049
Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049
Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049

Cat No. GTX01049

Host

Rabbit

Clonality

Monoclonal

Clone Name

SC06-78

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Observed MW

34 kDa. The observed M.W. is based on the following publication. PMID: 9670962|28575531

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

,HLADRA,HLADRA1,major histocompatibility complex, class II, DR alpha,HLA-DRA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus,Trans-Golgi network membrane,Endosome membrane,Lysosome membrane,Late endosome membrane

Background

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-DRA antibody [SC06-78] used in Western Blot (WB). GTX01049

GTX01049 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DRA antibody [SC06-78] (GTX01049) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 9670962 and PMID: 28575531

Anti-HLA-DRA antibody [SC06-78] used in Western Blot (WB). GTX01049

GTX01049 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DRA antibody [SC06-78] (GTX01049) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 9670962 and PMID: 28575531

Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049

GTX01049 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01049 HLA-DRA antibody [SC06-78].

Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049

GTX01049 IHC-P Image

IHC-P analysis of human liver tissue section using GTX01049 HLA-DRA antibody [SC06-78].

Anti-HLA-DRA antibody [SC06-78] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01049

GTX01049 ICC/IF Image

ICC/IF analysis of PFA-fixed B16-F1 cells using GTX01049 HLA-DRA antibody [SC06-78].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-HLA-DRA antibody [SC06-78] used in Flow cytometry (FACS). GTX01049

GTX01049 FACS Image

FACS analysis of Jurkat cells using GTX01049 HLA-DRA antibody [SC06-78].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049

GTX01049 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX01049 HLA-DRA antibody [SC06-78].

Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049

GTX01049 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX01049 HLA-DRA antibody [SC06-78].

Anti-HLA-DRA antibody [SC06-78] used in IHC (Paraffin sections) (IHC-P). GTX01049

GTX01049 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01049 HLA-DRA antibody [SC06-78].

REFERENCE

There are currently no references for HLA-DRA antibody [SC06-78] (GTX01049). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HLA-DRA antibody [SC06-78] (GTX01049). Be the first to share your experience with this product.
Package List Price ($)
$ 369