GeneTex
United States (US)

HLA-DRA antibody

GTX101865_39694_IHC_19011506_407.jpg
GTX101865_WB_19011506_847.jpg

Cat No. GTX101865

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

H1299
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DRA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ51114 antibody, HLA-DRA1 antibody, MLRW antibody, HLA-DRA antibody, MHC class II antigen DRA antibody, MHC cell surface glycoprotein antibody, histocompatibility antigen HLA-DR alpha antibody, HLA class II histocompatibility antigen, DR alpha chain antibody, major histocompatibility complex, class II, DR alpha antibody

Cellular Localization

Membrane

Background

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101865_39694_IHC_19011506_407.jpg

GTX101865 IHC-P Image

HLA-DRA antibody detects HLA-DRA protein at membrane on human Leukocytes in liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Leukocytes in liver carcinoma.
HLA-DRA antibody (GTX101865) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101865_WB_19011506_847.jpg

GTX101865 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX101865 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169