PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Positive Control
Liver: An at least moderate staining should be seen of sinusoids and Kupffer cells. Kidney: An at least moderate staining should be seen in glomerular capillaries.
Negative Control
Liver, kidney: HLA-DR staining must be absent normal in hepatocytes and in tubuli of completely normal kidney.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human HLA-DRB1 fragment
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
major histocompatibility complex, class II, DR beta 1 , DRB1 , HLA-DR1B , HLA-DRB , SS1
Cellular Localization
Cell membrane , Endoplasmic reticulum membrane , Golgi apparatus, trans-Golgi network membrane , Endosome membrane , Lysosome membrane , Late endosome membrane
Background
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457](/templates/default/js/lightbox/images/loading.gif "Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457")
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GTX04457 IHC-P Image
IHC-P analysis of human lung tissue section using GTX04457 HLA-DRB1 antibody [MSVA-478R] HistoMAX™.
Strong HLA DRB1 staining in alveolar macrophages HLA DRB1 immunohistochemistry.
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GTX04457 IHC-P Image
IHC-P analysis of human testis HLA DRB1 negative seminoma tissue section using GTX04457 HLA-DRB1 antibody [MSVA-478R] HistoMAX™.
HLA DRB1 negative seminoma showing HLA DRB1 staining of inflammatory cells and of small vessels HLA DRB1 immunohistochemistry.
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GTX04457 IHC-P Image
IHC-P analysis of human liver tissue section using GTX04457 HLA-DRB1 antibody [MSVA-478R] HistoMAX™.
Intense HLA DRB1 staining of inflammatory cells and of Kupffer cells HLA DRB1 immunohistochemistry.
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REFERENCE
There are currently no references for HLA-DRB1 antibody [MSVA-478R] HistoMAX™ (GTX04457). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HLA-DRB1 antibody [MSVA-478R] HistoMAX™ (GTX04457). Be the first to share your experience with this product.
![Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457](/upload/website/prouct_img/small/GTX04457/GTX04457_20230728_IHC-P_63_23072722_466.webp "Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457")
![Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457](/upload/website/prouct_img/small/GTX04457/GTX04457_20230728_IHC-P_193_23072723_202.webp "Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457")
![Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457](/upload/website/prouct_img/small/GTX04457/GTX04457_20230728_IHC-P_323_23072723_552.webp "Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457")
![Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457](/upload/website/prouct_img/normal/GTX04457/GTX04457_20230728_IHC-P_63_23072722_466.webp "Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457")
![Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457](/upload/website/prouct_img/normal/GTX04457/GTX04457_20230728_IHC-P_193_23072723_202.webp "Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457")
![Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457](/upload/website/prouct_img/normal/GTX04457/GTX04457_20230728_IHC-P_323_23072723_552.webp "Anti-HLA-DRB1 antibody [MSVA-478R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04457")