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HLA-DRB1 antibody [N1C3]

Anti-HLA-DRB1 antibody [N1C3] used in Western Blot (WB). GTX104919
Anti-HLA-DRB1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104919
Anti-HLA-DRB1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104919
Anti-HLA-DRB1 antibody [N1C3] used in Immunohistochemistry (IHC). GTX104919

Cat. No. GTX104919

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HLA-DRB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Product Note

Based on sequence homology, this antibody may cross react with HLA-DRB3, HLA-DRB4, or HLA-DRB5 protein.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DRB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616679

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DR beta 1 , DRB1 , HLA-DR1B , HLA-DRB , SS1

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome membrane,Lysosome membrane

Background

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-DRB1 antibody [N1C3] used in Western Blot (WB). GTX104919

GTX104919 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DRB1 antibody [N1C3] (GTX104919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HLA-DRB1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104919

GTX104919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using HLA-DRB1(GTX104919) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HLA-DRB1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104919

GTX104919 ICC/IF Image

HLA-DRB1 antibody [N1C3] detects HLA-DRB1 protein at cell membrane by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HLA-DRB1 stained by HLA-DRB1 antibody [N1C3] (GTX104919) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HLA-DRB1 antibody [N1C3] used in Immunohistochemistry (IHC). GTX104919

GTX104919 IHC Image

The data was published in the journal Aging (Albany NY) in 2017. PMID: 28283675

REFERENCE

Application Reference

Jeong HO et al. iScience 2022; 25 (11) : 105358 Cellular plasticity and immune microenvironment of malignant pleural effusion are associated with EGFR-TKI resistance in non-small-cell lung carcinoma.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Arase N et al. J Autoimmun 2021; 126 : 102774 Cell surface-expressed Ro52/IgG/HLA-DR complex is targeted by autoantibodies in patients with inflammatory myopathies.
Application : IHC-P
Reactivity : Human
Sreevalsan S et al. EMBO Rep 2020; 21 (12) : e50155 MLLT6 maintains PD-L1 expression and mediates tumor immune resistance.
Andres-Benito P et al. Aging (Albany NY) 2017; 9 (3) : 823-851 Amyotrophic lateral sclerosis, gene deregulation in the anterior horn of the spinal cord and frontal cortex area 8: implications in frontotemporal lobar degeneration.
Application : IHC-P
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169