GeneTex
United States (US)

HLA-DRB3 antibody

GTX105068_39904_IFA_19011506_205.jpg

Cat No. GTX105068

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-DRB3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HLA-DR3B antibody, HLA-DRB1 antibody, MGC117330 antibody, HLA-DRB3 antibody, MHC class II antigen DRB3 antibody, MHC class II antigen DR beta 3 chain antibody, MHC class II HLA-DR beta 3 chain antibody, HLA class II histocompatibility antigen, DR beta 3 chain antibody, human leucocyte antigen DRB3 antibody, HLA class II histocompatibility antigen, DRB1-7 beta chain antibody, major histocompatibility complex, class II, DR beta 3 antibody, DR7 antibody

Cellular Localization

Membrane

Background

HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105068_39904_IFA_19011506_205.jpg

GTX105068 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using HLA-DRB3(GTX105068) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169