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HLA-G antibody [2A12] (FITC)

Overexpression
Anti-HLA-G antibody [2A12] (FITC) used in Flow cytometry (FACS). GTX80289

Cat. No. GTX80289

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A12

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 μg ($349)
See all HLA-G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

The antibody 2A12 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allow to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.

Purification

Purified by size-exclusion chromatography

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HLAG , MHCG , major histocompatibility complex, class I, G , HLA-G

Cellular Localization

Cell membrane,Secreted

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-G antibody [2A12] (FITC) used in Flow cytometry (FACS). GTX80289

GTX80289 FACS Image

Overexpression

FACS analysis of HLA-G transfected cells (red, orange) compared with non-transfected cells (blue, green) using GTX80289 HLA-G antibody [2A12] (FITC).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349