APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| 10 μg/ml |
| 10 μg/ml |
| 2 μg/ml |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture : GTX80260, Detection : GTX00592-02. Tested sample : soluble HLA-G in HeLa overexpressing HLA-G5 cell lysate or supernatant.
|
Not tested in other applications.
Calculated MW
Product Note
The antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allow to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
HLAG , MHCG , major histocompatibility complex, class I, G , HLA-G
Cellular Localization
Cell membrane,Secreted
Background
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-HLA-G antibody [5A6G7] used in Western Blot (WB). GTX80260](/templates/default/js/lightbox/images/loading.gif "Anti-HLA-G antibody [5A6G7] used in Western Blot (WB). GTX80260")
|
GTX80260 WB Image
WB analysis of HLA-G stable transfectants (various splice variants) using GTX80260 HLA-G antibody [5A6G7].
Lane 1 : M8 cell line transfected with empty vector
Lane 2 : M8 cell line transfected with HLA-G1
Lane 3 : M8 cell line transfected with HLA-G5
Lane 4 : M8 cell line transfected with HLA-G6
|
REFERENCE
There are currently no references for HLA-G antibody [5A6G7] (GTX80260). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HLA-G antibody [5A6G7] (GTX80260). Be the first to share your experience with this product.
![Anti-HLA-G antibody [5A6G7] used in Western Blot (WB). GTX80260](/upload/website/prouct_img/small/GTX80260/GTX80260_20191028_WB_1_w_23061322_595.webp "Anti-HLA-G antibody [5A6G7] used in Western Blot (WB). GTX80260")
![Anti-HLA-G antibody [5A6G7] used in Western Blot (WB). GTX80260](/upload/website/prouct_img/normal/GTX80260/GTX80260_20191028_WB_1_w_23061322_595.webp "Anti-HLA-G antibody [5A6G7] used in Western Blot (WB). GTX80260")