GeneTex
  • Country / Location Selection

United States (US)

HLA-G antibody [5A6G7]

Anti-HLA-G antibody [5A6G7] used in Western Blot (WB). GTX80260

Cat No. GTX80260

Host

Mouse

Clonality

Monoclonal

Clone Name

5A6G7

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, Sandwich ELISA

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 10 μg/ml
IHC-Fr 10 μg/ml
FACS 2 μg/ml
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX80260, Detection : GTX00592-02. Tested sample : soluble HLA-G in HeLa overexpressing HLA-G5 cell lysate or supernatant.

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

The antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allow to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HLAG , MHCG , major histocompatibility complex, class I, G , HLA-G

Cellular Localization

Cell membrane,Secreted

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-G antibody [5A6G7] used in Western Blot (WB). GTX80260

GTX80260 WB Image

WB analysis of HLA-G stable transfectants (various splice variants) using GTX80260 HLA-G antibody [5A6G7].
Lane 1 : M8 cell line transfected with empty vector
Lane 2 : M8 cell line transfected with HLA-G1
Lane 3 : M8 cell line transfected with HLA-G5
Lane 4 : M8 cell line transfected with HLA-G6

REFERENCE

There are currently no references for HLA-G antibody [5A6G7] (GTX80260). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HLA-G antibody [5A6G7] (GTX80260). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379