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HLA-G antibody [87G] (Low Endotoxin)

Anti-HLA-G antibody [87G] (Low Endotoxin) used in Functional Assay (Functional Assay). GTX80252

Cat. No. GTX80252

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 87G

Isotype

 IgG2a

Application

 IHC-Fr, FACS, ELISA, Functional Assay

Reactivity

 Human
Package
100 μg ($399)
See all HLA-G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). The antibody 87G blocks interaction of HLA-G with inhibitory receptors.
We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.

Purification

Protein A purified

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_11179010

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HLAG , MHCG , major histocompatibility complex, class I, G , HLA-G

Cellular Localization

Cell membrane,Secreted

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-G antibody [87G] (Low Endotoxin) used in Functional Assay (Functional Assay). GTX80252

GTX80252 Functional Assay Image

Effect on cytolytical activity of human polyclonal NK cells on M8 cells transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6). Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis.
Blocking antibodies -
Column 1-2 : None
Column 3 : Isotype mouse IgG2a control
Column 4 : GTX80252 anti-human HLA-G (87G) purified
Column 5 : anti-human HLA-G (87G) F(ab’)2 fragment
Column 6 : anti-human HLA-G (MEM-G/9)

REFERENCE

Application Reference

Yang S et al. Cytotherapy 2020; (Epub) Umbilical cord blood-derived mesenchymal stromal cells promote myeloid-derived suppressor cell proliferation by secreting HLA-G to reduce acute graft-versus-host disease after hematopoietic stem cell transplantation.
Application : Functional Assay
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations