GeneTex
  • Country / Location Selection

United States (US)

HLA-G antibody [MEM-G/9] (Azide Free)

Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Western Blot (WB). GTX78332
Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Flow cytometry (FACS). GTX78332

Cat No. GTX78332

Host

Mouse

Clonality

Monoclonal

Clone Name

MEM-G/9

Isotype

IgG1

Application

WB, ICC/IF, IHC-Fr, FACS, IP, ELISA

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

This antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_625691

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HLAG , MHCG , major histocompatibility complex, class I, G , HLA-G

Cellular Localization

Cell membrane,Secreted

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Western Blot (WB). GTX78332

GTX78332 WB Image

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) (GTX78332)and protein G. HLA-G was detected by anti-human HLA-G and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Flow cytometry (FACS). GTX78332

GTX78332 FACS Image

Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.

REFERENCE

There are currently no references for HLA-G antibody [MEM-G/9] (Azide Free) (GTX78332). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HLA-G antibody [MEM-G/9] (Azide Free) (GTX78332). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 379
Custom Conjugations