This antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
We do not recommend use of this product for Mouse samples.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Purification
Protein A purified
Conjugation
Unconjugated
RRID
AB_625691
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
HLAG , MHCG , major histocompatibility complex, class I, G , HLA-G
Cellular Localization
Cell membrane,Secreted
Background
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) (GTX78332)and protein G. HLA-G was detected by anti-human HLA-G and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
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GTX78332 FACS Image
Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.
![Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Western Blot (WB). GTX78332](/upload/website/prouct_img/small/GTX78332/HLA-G-antibody-MEM-G9__GTX78332-2_w_23061322_210.webp "Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Western Blot (WB). GTX78332")
![Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Flow cytometry (FACS). GTX78332](/upload/website/prouct_img/small/GTX78332/HLA-G-antibody-MEM-G9__GTX78332-1_w_23061322_890.webp "Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Flow cytometry (FACS). GTX78332")
![Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Western Blot (WB). GTX78332](/upload/website/prouct_img/normal/GTX78332/HLA-G-antibody-MEM-G9__GTX78332-2_w_23061322_210.webp "Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Western Blot (WB). GTX78332")
![Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Flow cytometry (FACS). GTX78332](/upload/website/prouct_img/normal/GTX78332/HLA-G-antibody-MEM-G9__GTX78332-1_w_23061322_890.webp "Anti-HLA-G antibody [MEM-G/9] (Azide Free) used in Flow cytometry (FACS). GTX78332")