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HLA-G antibody [MEM-G/9] (Biotin)

Anti-HLA-G antibody [MEM-G/9] (Biotin) used in Flow cytometry (FACS). GTX78333
Anti-HLA-G antibody [MEM-G/9] (Biotin) used in Western Blot (WB). GTX78333

Cat No. GTX78333

Host

Mouse

Clonality

Monoclonal

Clone Name

MEM-G/9

Isotype

IgG1

Application

WB, ICC/IF, IHC-Fr, FACS, IP, ELISA

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

This antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Purification

Purified IgG

Conjugation

Biotin
The reagent is free of unconjugated biotin.

RRID

AB_625687

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HLAG , MHCG , major histocompatibility complex, class I, G , HLA-G

Cellular Localization

Cell membrane,Secreted

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-G antibody [MEM-G/9] (Biotin) used in Flow cytometry (FACS). GTX78333

GTX78333 FACS Image

Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.

Anti-HLA-G antibody [MEM-G/9] (Biotin) used in Western Blot (WB). GTX78333

GTX78333 WB Image

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) (GTX78332)and protein G. HLA-G was detected by anti-human HLA-G and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379