FACS: Use at a dilution of 1/500. Optimal dilutions/concentrations should be determined by the end user.
Reacts with native form of human HLA-G on the cell surface. Specific reaction was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA.
Phosphate buffered saline pH 7.4 containing 15mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant human HLA G refolded with beta 2 microglobulin and peptide.
Fluorescein isothiocyanate (FITC)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Major Histocompatibility Complex, Class I, G,Mhc-G,Hla-G
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq]
GTX27904 IP Image
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) (GTX27758) and protein G. HLA-G was detected by anti-human HLA-G (4H84) (GTX80268) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
GTX27904 FACS Image
Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC (GTX79919 ) and anti-human HLA-G (MEM-G/11) FITC (GTX78370).