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HLA-G antibody [MEM-G/9]

Anti-HLA-G antibody [MEM-G/9] used in Western Blot (WB). GTX27758
Anti-HLA-G antibody [MEM-G/9] used in Flow cytometry (FACS). GTX27758

Cat No. GTX27758

Host Mouse
Clonality Monoclonal
Clone Name MEM-G/9
Isotype IgG1
Application WB, IHC-Fr, FACS, ELISA
Reactivity Human, Primate
Package
100μg ($319)
APPLICATION

Application Note

ELISA: Use at an assay dependent concentration. FACS: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

38 kDa. ( Note )

Specificity/Sensitivity

Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA.This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Major Histocompatibility Complex, Class I, G,Mhc-G,Hla-G

Cellular Localization

Cell Membrane

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HLA-G antibody [MEM-G/9] used in Western Blot (WB). GTX27758

GTX27758 WB Image

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) (GTX27758) and protein G. HLA-G was detected by anti-human HLA-G (4H84) (GTX80268) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

Anti-HLA-G antibody [MEM-G/9] used in Flow cytometry (FACS). GTX27758

GTX27758 FACS Image

Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC (GTX79919 ) and anti-human HLA-G (MEM-G/11) FITC (GTX78370).

REFERENCE
There are currently no references for HLA-G antibody [MEM-G/9] (GTX27758).
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REVIEW
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Package List Price ($)
$ 319