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HLA-G antibody

Anti-HLA-G antibody used in IHC (Paraffin sections) (IHC-P). GTX112713
Anti-HLA-G antibody used in IHC (Paraffin sections) (IHC-P). GTX112713
Anti-HLA-G antibody used in Western Blot (WB). GTX112713
Anti-HLA-G antibody used in IHC (Paraffin sections) (IHC-P). GTX112713

Cat. No. GTX112713

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HLA-G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA-G. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166686

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class I, G , MHC-G

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HLA-G antibody used in IHC (Paraffin sections) (IHC-P). GTX112713

GTX112713 IHC-P Image

HLA-G antibody detects HLA-G protein at cytoplasm and membrane in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
HLA-G antibody (GTX112713) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HLA-G antibody used in IHC (Paraffin sections) (IHC-P). GTX112713

GTX112713 IHC-P Image

HLA-G antibody detects HLA-G protein at cytoplasm and membrane in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
HLA-G antibody (GTX112713) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HLA-G antibody used in Western Blot (WB). GTX112713

GTX112713 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-G antibody (GTX112713) diluted at 1:500.

Anti-HLA-G antibody used in IHC (Paraffin sections) (IHC-P). GTX112713

GTX112713 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using HLA-G(GTX112713) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169